Laboratory Mouse Procedural Techniques : Manual and DVD by John J. Bogdanske, Scott Hubbard-Van Stelle, Margaret Rankin

By John J. Bogdanske, Scott Hubbard-Van Stelle, Margaret Rankin Riley, Beth M. Schiffman

DVD textual content and Voice-over Mouse Handling/TransferMouse RestraintOral GavageOne-Handed Injection TechniqueIntraperitoneal Injection (IP) - One personIntraperitoneal Injection (IP) - One-Person Towel MethodSubcutaneous Injection (SQ)Subcutaneous Injection (SQ) - One-Person Towel MethodMaxillary BleedPedal Vein Blood DrawSaphenous Vein Blood DrawTail Vein InjectionRetro-Orbital Bleed - Anesthesia RequiredRetro-Orbital Read more...

summary: DVD textual content and Voice-over Mouse Handling/TransferMouse RestraintOral GavageOne-Handed Injection TechniqueIntraperitoneal Injection (IP) - One personIntraperitoneal Injection (IP) - One-Person Towel MethodSubcutaneous Injection (SQ)Subcutaneous Injection (SQ) - One-Person Towel MethodMaxillary BleedPedal Vein Blood DrawSaphenous Vein Blood DrawTail Vein InjectionRetro-Orbital Bleed - Anesthesia RequiredRetro-Orbital Injections - Anesthesia RequiredEar NotchingEar TaggingMouse Procedural approach Hand-outsIntraperitoneal Injection (IP)Subcutaneous Injection (SQ)Maxillary BleedOral GavagePedal Vei

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The ointment will allow the blood to bead up on the skin. Using a 27-gauge needle, gently poke into the vein, taking care not to pierce completely through it because this will result in blood accumulating under the skin. As the blood beads up, you can use a heparinized capillary tube to collect the blood. When finished, release the tourniquet and apply pressure with gauze to stop the bleeding. 23 24 laboratory mouse procedural techniques: manual and DVD recommended supplies • Heparinized capillary tube • Clippers • Artificial tears (petroleum jelly) • Tourniquet • 27-gauge needle • Gauze Voice-Over 1: Begin by clipping the lateral surface of the mouse’s hind leg.

Check the markings regularly to ensure the mice can be identified accurately, especially if mice are group housed. During fights, the mice could rip each others’ ears, making identification impossible. Removed tissue can be used for genotyping, possibly replacing the need for a tail biopsy. 16 ear tagging Ear tagging is a long-term identification method that can be performed quickly with little pain or distress. Mice are restrained, and using an ear tag applicator, a uniquely numbered tag is placed in the lower one-third of the ear.

There will be virtually no resistance when correctly injecting into the vein. If a whitish bleb or bubble appears under the skin, stop injecting, remove the needle, and choose a site closer to the base of the tail. recommended supplies • 1- or 3-ml syringe • 27- to 30-gauge needles • Restraint tube • Gel pack 25 26 laboratory mouse procedural techniques: manual and DVD Voice-Over 1: Begin by warming a gel pack in a microwave for approximately 30 seconds. Touch the pack to your wrist to ensure it is not too hot before wrapping it around the mouse’s tail; warming the tail will cause the tail veins to dilate.

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